Effects of Temperature-Induced Changes in Membrane Composition on Transformation Efficiency in E. coli DH5α
نویسندگان
چکیده
One model of transformation proposed that heat shock destabilizes the cell membrane and allows for plasmid uptake. As lipid composition might also affect the stability of the cell membrane, we hypothesized that a lower growth temperature would alter membrane lipid composition so as to increase transformation efficiency after heat shock. Cells were grown at 20°C and 37°C and treated with calcium chloride to induce competency. The transformation efficiencies yielded considerable variation. We were unable to conclude an effect of growth temperature on transformation efficiency. Statistical analysis determined that increased plasmid concentration negatively impacted cell survival.
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Influences of growth temperature and preparation of competent cells on efficiency of chemically-induced transformation in Escherichia coli DH5α
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